xQuant™ dsDNA HS Assay Kit make DNA quantitation easy and accurate, for the fluorescence quantitative detection of double-stranded DNA ( dsDNA ). This kit includes pre-mixed working solution ( with fluorescent dye ) and dsDNA standards. To perform the assay, simply dilute your sample (any volume from 1 ~ 20 μL is acceptable) into the 1× working solution provided, then read the concentration using a Qubit™ Fluorometer. It allows accurate quantification of dsDNA samples at concentration from 10 pg/μL to 100 ng/μL. The fluorescent signal of this product is stable at room temperature for 3 hours, also has good tolserance for some conventional contaminants such as ssDNA, RNA , salts , free nucleotides , proteins , solvents , detergent , etc . It is easy to operate. Add the appropriate amount of sample directly to the working solution and detect by Qubit fluorometer.